Split FASTA divides FASTA sequence records into smaller FASTA sequences of the size you specify. An optional overlap value can be used to create sequences that overlap.

Paste the FASTA sequences into the text area below. Input limit is 500000 characters.

  • The new sequences should be bases long.
  • The new sequences should overlap by bases.


P. Stothard. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. Biotechniques 2000; 28: 1102, 1104.