pcr_primer_stats
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PCR Primer Stats accepts a list of PCR primer sequences and returns a report describing the properties of each primer, including melting temperature, percent GC content, and PCR suitability. Use PCR Primer Stats to evaluate potential PCR primers.

Paste the raw sequence or one or more FASTA sequences into the text area below. Input limit is 5000 characters. The maximum accepted primer length is 50 bases.

Reference:

P. Stothard. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. Biotechniques 2000; 28: 1102, 1104.
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