Mutate for Digest accepts a DNA sequence as input and searches for regions that can easily be mutated to create a restriction site of interest. The program also displays protein translations so that you can see which reading frames are altered by the proposed mutations. Use Mutate for Digest to find sequences that can be converted to a useful restriction site using PCR or site-directed mutagenesis.

Paste a raw sequence or one or more FASTA sequences into the text area below. Input limit is 10000 characters.

  • Search for future sites.
  • Show bases per line.
  • Show the translation for reading frame
  • Use the genetic code.


P. Stothard. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. Biotechniques 2000; 28: 1102, 1104.