Multi Rev Trans accepts a protein alignment and uses a codon usage table to generate a degenerate DNA coding sequence. The program also returns a graph that can be used to find regions of minimal degeneracy at the nucleotide level. Use Multi Rev Trans when designing PCR primers to anneal to an unsequenced coding sequence from a related species.

Paste the protein alignment in FASTA or GDE format into the text area below. Input limit is 20000 characters.

Enter the codon table you wish to use (in GCG format). The default codon usage table was generated using all the E. coli coding sequences in GenBank. It was obtained from the Codon Usage Database.


P. Stothard. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. Biotechniques 2000; 28: 1102, 1104.