Multi Rev Trans accepts a protein alignment and uses a codon usage table to generate a degenerate DNA coding sequence. The program also returns a graph that can be used to find regions of minimal degeneracy at the nucleotide level. Use Multi Rev Trans when designing PCR primers to anneal to an unsequenced coding sequence from a related species.
Reference:
P. Stothard. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. Biotechniques 2000; 28: 1102, 1104.